These files are a description on how to build and program a high precision pressure pump to control tissue distension in Ussing chambers. In addition there is a video clip to show the ramp tissues distension
Study Purpose: Microfluidics has become a critical tool in research across the biological, chemical, and physical sciences. One important component of microfluidic experimentation is a stable fluid handling system capable of accurately providing an inlet flow rate or inlet pressure.
Data Collected: The Ussing Chamber Pressure Pump is a syringe pump that injects volume (air or liquid) into a closed Ussing chamber until a predefined pressure is reached. The design is based upon several publications (Lake et al., 2017; Wijnen et al., 2014). The syringe is operated by a stepper motor that is controlled by an Arduino microcontroller. For the pump controller, the Arduino was combined with the “EasyDriver” stepper motor driver by Brian Schmalz (Schmalz) and a pressure sensor (PendoTECH, Single Use Pressure Sensor, PRESS-S-000). The “Arduino IDE” (“Integrated Development Environment”) can be downloaded from: https://www.arduino.cc/en/Main/Software
Primary Conclusion: The IDE is necessary to develop programs and load them onto the Arduino hardware. It also contains a “Serial Monitor” that allows communication with the Arduino (Input of parameters/display of messages or measurements).
Experimental design: This is not applicable as this is technology development.
Completeness: This dataset is complete.
Subjects & Samples: None specified. However, the device was tested on a male Dunkin-Hartley guinea pig. A movie showing a test of the device with guinea pig tissue is included in docs folder "Submucosa-Mucosa preparation of the colon from a male Dunkin-Hartley guinea pig".
Primary vs derivative data: There is no raw data or derived data available in this dataset. The code file is named pressure11, and it is sitting in the documents folder. While the schematic is available as a pdf file, the instructions to 3D print the device is only available in the referenced below publication.
Important note: This work is reported to have been published in the following papers: J Vis Exp. 2018 Aug 30;(138). doi: 10.3791/57532. "Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications." Chen MC1, Lake JR1, Heyde KC2, Ruder WC3. PMID:30222163 Wijnen B, Hunt EJ, Anzalone GC, Pearce JM (2014) Open-Source Syringe Pump Library. PLoS ONE 9(9): e107216. https://doi.org/10.1371/journal.pone.0107216
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