Spatial distribution and morphometric characterization of vagal efferents associated with the myenteric plexus of the rat stomach

Terry Powley
,
Robert Phillips
,
Deborah Jaffey
,
Bartek Rajwa
,
Jennifer McAdams
,
Elizabeth Baronowsky
,
Logan Chesney
,
Diana Black
,
Charlene Evans

Spatial distribution and morphometric characterization of vagal efferents associated with the myenteric plexus of the rat stomach.

Updated on May 9, 2020 (Version 3, Revision 1)

Corresponding Contributor:

Terry Powley
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Dataset Overview

Study Purpose As part of the SPARC project, we are interested in understanding the correlations and causative relations between anatomical and functional characteristics of the stomach.

Data Collected Preganglionic efferents are one type of vagal innervation that is associated with the stomach. To characterize preganglionic efferents better, rats were given injections of dextran biotin in the dmnX, and, after tracer transport, stomach whole mounts were collected. Specimens were processed for avidin-biotin permanent labeling, and neurites were then digitized for morphometry and mapping using Neurolucida neural tracing software. This dataset (to date) consists of 131 XML files (~1MB to ~20 MB each) corresponding to preganglionic efferents traced from Sprague-Dawley young adult male rat stomachs. Each tracing was analyzed in Neurolucida Explorer to define key neurite metrics such as area of innervation, average branch length etc. In addition, the location of the first branch point for each arbor was measured and used to construct a map of efferent locations across the stomach. In parallel activities, we are assessing the potential correlation between efferent spatial distributions and the efficacy of stimulation locations for inducing gastric motility. In addition to the digitized tracing files, the dataset also includes: (1) an Excel spreadsheet containing morphometric data, (2) a set of whole mount photographs (jpg) used to register individual stomach contours to a standard stomach contour for generation of the 2D map; (3) a folder of 16 PNG images together with an html file that will display an interactive 2D map of neurite locations based on the associated PNG files.

Primary Conclusion This dataset represents a detailed anatomical 2D map of the standard rat stomach as well as an interactive 2D map of neurite locations.


Curator's Notes

Experimental design: The data is separated based on dorsal and ventral whole mounts from 70 male, adult, Sprague-Dawley rats. Dorsal and ventral stomach whole mounts were obtained and processed for histology. The tissues are counterstained with cuprolinic blue, nNOS+ or no counterstain.

Completeness: This dataset is part of a larger study, with two related datasets available.

Subjects & Samples: The dataset used adult male Sprague-Dawley rats, between 47 and 116 days old.

Primary vs derivative data: The raw data is formatted as xml. The series of images that were used to register individual images whole mount contours are contained in the source folder. There is a cumulative excel file of morphometric data in the derivatives folder.

Important note: Most of the images that are "human" friendly, including the 2D maps are in the documents folder. The primary data folder is filled with xml flies. This dataset is has two similarly named sister datasets: "Spatial distribution and morphometric characterization of vagal afferents associated with the myenteric plexus of the rat stomach" (N:dataset:d484110a-e6e3-4574-aab2-418703c978e2), and “Spatial distribution and morphometric characterization of vagal afferents (intramuscular arrays (IMAs)) within the longitudinal and circular muscle layers of the rat stomach” (N:dataset:e4bfb720-a367-42ab-92dd-31fd7eefb82e ) which use sister subjects but examine other types of vagal innervation.

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About this dataset

Publishing history

July 12, 2019
Originally Published
May 9, 2020 (Version 3)
Last Updated

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