A normal male rat heart was fixed and whole-mount diffusion stained with Cresyl Echt Violet to visualize ICN neurons. The whole heart was sectioned and imaged with a Knife Edge Scanning Microscope (KESM) to produce an image stack. Cardiac anatomy was segmented and single neurons were mapped in the image stack with the Tissue Mapper software. The annotated heart anatomy and distribution of single neurons were then visualized in a 3D reconstructed model of the heart.
1 - 4 of 4 files