Phrenic Nerve Immunohistochemistry

Gordon Mitchell
,
Elisa Gonzalez-Rothi
,
Donald Bolser
,
Latoya Allen
,
Marissa Ciesla
,
Yasin Seven

Immunohistochemistry of phrenic nerve afferents in acute intermittent hypoxia and hemisection injury conditioned rats

Updated on May 18, 2020 (Version 1)

Corresponding Contributor:

Gordon Mitchell
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Dataset Overview

Study purpose: The goal of the study was to examine phrenic nerve afferents in acute intermittent hypoxia and hemisection injury conditioned rats.

Data collection: Immunohistochemical studies were conducted on spinal cord sections retrogradely labeled with β‐subunit of cholera toxin (CTB) to trace phrenic motor neurons. Sections were examined for the expression of CTB, spinal adenosine 2A (a2), serotonin (5-HT), serotonin 2A Receptor (5-HT2AR), and Serotonin 7 Receptor (5-HT7).

Primary conclusion: None stated


Curator's Notes

Experimental Design: Rats were assigned to 2 experimental groups by injury: (1) intact spinal cord (INTACT) and (2) Cervical C2 hemi-section injury (C2HX). Each group underwent long-term normoxia (Nx), daily acute intermittent hypoxia (dAIH), chronic intermittent hypoxia simulating mild sleep apnea (apnea-hypopnea equivalent = 6 episodes/hour) (IH-5/5), and chronic intermittent hypoxia simulating moderate sleep apnea (apnea-hypopnea equivalent = 15 episodes/hour) (IH-2/2). Phrenic motor neurons were retrogradely labeled via intrapleural injections of the β‐subunit of cholera toxin (CTB). Spinal tissue was removed, fixed, sectioned, and stained for spinal adenosine 2A (a2), serotonin (5-HT), Serotonin 2A Receptor (5-HT2AR) and Serotonin 7 Receptor (5-HT7).

Completeness: Complete

Subjects & Samples: Adult male Sprague-Dawley rats (n=105), age unknown, were used for this study. Spinal cord samples with retrogradely labeled phrenic motor neurons were stained for spinal adenosine 2A (a2), serotonin (5-HT), Serotonin 2A Receptor (5-HT2AR), and Serotonin 7 Receptor (5-HT7).

Primary vs derivative data: The primary and derivative folders are organized by subject identification, then by region stained, orientation, the antibody used, section number, and magnification. The images are tif formatted. There is not a derivative folder.

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Publishing history

May 18, 2020
Originally Published
May 18, 2020 (Version 1)
Last Updated

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