Characterises EECs in the human gastric fundus and corpus using immunohistochemistry and high resolution 3-D microscopy.
Study purpose: The study aims to characterize the endocrine cells in the mucosa of the stomach in humans, in terms of their hormone content, morphology and relationship to other cells. It provides a basis for future studies looking at how these cells change in disease states.
Data collected: Human gastric fundus and corpus samples were studied using immunohistochemistry and super-resolution microscopy. Endocrine cells (EECs) in the gastric mucosa were studied using immunohistochemistry with antibodies to ghrelin, serotonin (5-HT), somatostatin, peptide YY (PYY), glucagon-like peptide 1, calbindin, gastrin and pancreastatin, the latter as a marker of enterochromaffin-like (ECL) cells. Cells were counted in various regions of the mucosa in large tile scan confocal images.
Primary conclusion: Four major chemically defined EEC types were observed in the acid producing regions of the human stomach, each with distinct morphologies and relationships to other mucosal cells.
Experimental Design: Adult male (n=1) and female (n=3) stomach tissue removed patients undergoing gastric sleeve surgery for obesity as used for this study. The gastric fundas and corpus was collected for each subject, fixed, sectioned, and stained for the presence of ghrelin, serotonin (5-HT), somatostatin, peptide YY (PYY), glucagon-like peptide 1, calbindin, gastrin, and pancreastatin.
Subjects & Samples: The gastric fundus and corpus were sampled from adult human patients undergoing gastric sleeve surgery for obesity. Immunohistochemistry studies were conducted to identify ghrelin, serotonin (5-HT), somatostatin, peptide YY (PYY), glucagon-like peptide 1, calbindin, gastrin and pancreastatin.
Primary vs. derivative data: Primary and derivative folders are organized by subject identification and sampled tissue (fundus or corpus). The primary folder contains quantification analysis(xlsx), image (tif, czi), the region of interest definitions for each cell (zip), and cell count (xml) for each sample. The derivative folder consists of tif and cdr formatted images.
(1) Doc Folder. This folder contains a species comparison of gastric EEC's.
Code Availability: ImageJ macro file developed in Fiji for defining mucosal regions (bottom, top, and middle)
The subjects file documents 4 human patients while the protocol states that 6 subjects were used for this study.
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