Human Vagus Nerve Stained with Masson's Trichrome

Nicole A. Pelot
,
J. Ashley Ezzell
,
Gabriel B. Goldhagen
,
Eric Musselman
,
Jake E. Cariello
,
Kara A. Clissold
,
Warren M. Grill

Micrographs of cross sections of cervical and abdominal human vagus nerve stained with Masson's trichrome.

Updated on July 17, 2020 (Version 2)

Corresponding Contributor:

Nicole Pelot
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Dataset Overview

Study purpose: To collect samples of human vagus nerves at the cervical and subdiaphragmatic levels and provide Masson’s trichrome-stained cross sections

Data collection: We collected 18 left cervical, 19 right cervical, 16 anterior subdiaphragmatic, and 16 posterior subdiaphragmatic vagus nerve samples from embalmed human cadavers and placed them in 4% PFA. Following paraffin embedding, we stained transverse cross sections with Masson’s trichrome. The dataset contains nd2 (filetype for Nikon’s NIS-Elements software) and TIFF files.

Primary conclusion: None stated


Curator's Notes

Experimental Design: Cervical and/or subdiphragmatic vagus nerves were sampled from adult embalmed human cadavers (male and female), and each sample underwent paraffin embedding, transverse sectioning, and Masson’s trichrome staining.

Completeness: This dataset is part of a larger study "Quantified vagus nerve morphology across species".

Subjects & Samples: Adult male (n=15) and female (n=15) embalmed human cadavers were used for this study, 54 - 90+ years old. The dataset contains 18 left cervical, 19 right cervical, 16 anterior subdiaphragmatic, and 16 posterior subdiaphragmatic human vagus nerve samples.

Primary vs derivative data: Data in the primary and derived files are divided by subject identification, then sample number (listed in sample file). The primary folder contains nd2 and TIFF images. There is no derivative folder.

Important notes: (1) Fiji software can be used to open nd2 files (https://imagej.net/Fiji/Downloads) > Open Fiji > Plugins (top of the screen) > Bio-Formats > Bio-Formats Importer > Select the file.

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About this dataset

Publishing history

February 28, 2020
Originally Published
July 17, 2020 (Version 2)
Last Updated

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