Binary traces from segmentation of cross sections of cervical and subdiaphragmatic pig vagus nerves stained with Masson's trichrome. Quantified effective nerve diameter, effective fascicle diameter, and number of fascicles.
Study purpose: To quantify pig vagus nerve morphology
Data collection: Using micrographs of pig vagus nerve cross sections that were stained with Masson’s trichrome, we quantified effective nerve and fascicle diameters, as well as the number of fascicles at the cervical and subdiaphragmatic levels. These morphological data provide neural anatomical information, as well as foundational knowledge for computational and preclinical studies of vagus nerve stimulation. The dataset contains TIFF files for 9 left cervical and 9 anterior subdiaphragmatic pig vagus nerve samples, providing segmented traces of the fascicles and nerves.
Primary conclusion: None stated
Experimental Design: We segmented Masson’s trichrome micrographs of 9 left cervical and 9 anterior subdiaphragmatic pig vagus nerves using Nikon’s NIS-Elements to identify fascicular (inner perineurial) and neural boundaries. Using the resulting binary traces, we quantified the effective nerve and fascicle diameters, as well as the number of fascicles.
Completeness: This dataset is part of a larger study "Quantified vagus nerve morphology across species".
Subjects & Samples: Adult male (n=3) and female (n=8) domestic pink pigs were used for this study, 10 - 15 weeks old.
Primary vs derivative data: Data in the primary and derivative folders are divided by subject number (listed in subject file), then sample number (listed in sample file). The primary folder contains TIFF images of the original histology (for reference; not used in analyses), and segmentations of the histology resulting in TIFF images of the binary traces of the fascicles and nerves (loaded and analyzed by Matlab scripts in the "code" folder). The derivative folder contains a .mat file with the morphology metrics output by the code, as well as xml and jp2 versions of the image files; Neurolucida 360 from MBF Bioscience was used to convert the binary traces into xml file format to overlay with the original Masson’s trichrome nd2 micrographs (in prior dataset) and then to convert the images to jp2 extension.
Code Availability: In the "code" folder, Matlab code is used to load the segmented/binary TIFF images, analyze the images, and plot the resulting morphology metrics. Filepaths in the code are set to work as-is, in-place in the dataset.
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