Utilizes pseudorabies virus (PRV) retrograde tracing in combination w/reporter mice, iDISCO, & confocal/light-sheet microscopy to identify the location of pre- & post-ganglionic neurons and nerves that selectively innervate the iBAT in the mouse.
Study purpose: To identify the location of pre- and post-ganglionic neurons as well as nerves that selectively innervate the interscapular brown adipose tissue, iBAT, in the mouse.
Data collection: Pseudorabies virus was injected into interscapular brown adipose tissue, iBAT, and then immunostain for tyrosine hydroxylase peptide (TH) and PRV-GFP and, iDISCO. Light-sheet microscopy and confocal microscopy were used to collect high-resolution image files, qualitative anatomical identification, quantitative cell counts and videos.
Primary conclusion: None stated
Experimental Design: Adult male (n=2) and female (n=9) transgenic mice from 3 strain of mice (B6.129X1-Thtm1(cre)Te/Kieng, C57BL/6J and STOCK Slc32a1tm2(cre)Lowl/J) were used for this study. The interscapular brown adipose tissue (iBAT) received a unilateral injection with a green fluorescent protein (GFP) expressing Pseudorabies virus (PRV). iBAT and entire spinal cord were removed, sectioned and stained against GFP and Tyrosine Hydroxylase.
Completeness: This dataset is part of a larger study.
Subjects & Samples: Male and female adult mice ages between 2 months to 6 months old. were used. Interscapular brown adipose tissue (iBAT) and spinal cord were removed from all 3 strains of mice. Immunohistochemistry studies were completed on the dorsal upper spine, dorsal lower spine, ventral upper spine, ventral lower spine and the preganglionic intermediolateral (IML) against tyrosine hydroxylase and GFP.
Primary vs derivative data: Primary and derivative folders are organized by subject identification, then by region stained. The primary folder contains raw (ims) and color corrected (tif) images of the tissue. The derived data contains AVI video files of the samples.
Important notes: The protocol is very helpful and detailed.
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